FIG 5.

Circular map visualizing chromosomal nucleotide similarities between strains of the circulating lineage and unrelated reference/KPC-negative E. cloacae genomes. The outer two rings represent the genome of strain NDA, with coding regions in forward and reverse orientations and tRNA genes colored purple. The next 28 rings depict nucleotide similarities between strain NDA and other sequenced E. cloacae isolates belonging to the circulating lineage (some strains were excluded to eliminate redundancy). Similarities were calculated using nucleotide BLAST in 1,000-bp increments across the chromosome. The remaining rings are unrelated E. cloacae reference/KPC-negative strains in the following order: MNCRE15, MNCRE55, ATCC 13047, MNCRE04, MNCRE16, MNCRE03, MNCRE20, MNCRE14, MNCRE46, and MNCRE47. The innermost ring depicts deviation from the average G+C content for the chromosome, with gold representing higher G+C content and maroon representing lower G+C content. Asterisks depict regions of interest, as defined in Table 3. The legend for BLAST similarity colors is in the center of the circle.