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Immunoblot of the four IMP enzymes investigated. Lanes 1 to 4, crude cell lysates; lane 5, 50 ng purified IMP-1; lanes 6 to 9, whole cells. Lanes 1 and 6, IMP-1 (V67); lanes 2 and 7, IMP-10 (F67); lanes 3 and 8, IMP-1-V67I; lanes 4 and 9, IMP-1-V67A. DnaK was used as a constitutively expressed control.
