TABLE 1.
RT-PCR gene expression analysis with strains of B. cenocepacia J2315
| Strain | Expression relative to that of the WTa |
||
|---|---|---|---|
| ampCb | penB | penR (ampRc) | |
| WT and mutants | |||
| WT | 1 | 1 | 1 |
| M-Dup1d | 8,724.7 ± 2,861.7 | 750.3 ± 413.2 | 1.1 ± 0.2 |
| M-Del6 | 8,110.0 ± 2,846.1 | 493.8 ± 82.8 | 0.8 ± 0.2 |
| M-In1 | 5,494.6 ± 1,780.3 | 407.2 ± 24.8 | 0.7 ± 0.2 |
| M-Sub5 | 2,489.1 ± 640.4 | 243.5 ± 4.7 | 0.5 ± 0.1 |
| M-Sub8 | 1,999.0 ± 666.6 | 241.4 ± 38.7 | 0.4 ± 0.2 |
| M-Sub14 | 1,508.0 ± 441.9 | 234.1 ± 18.7 | 0.4 ± 0.1 |
| M-Ext2 | 600.4 ± 131.7 | 135.5 ± 20.5 | 0.4 ± 0.0 |
| WT with inductione using: | |||
| AMX | 62.2 ± 8.9 | 40.4 ± 2.1 | 0.6 ± 0.1 |
| CAZ | 12.4 ± 1.0 | 14.3 ± 1.6 | 0.8 ± 0.1 |
| CTXM | 23.4 ± 6.1 | 38.6 ± 3.6 | 0.4 ± 0.1 |
| MER | 409.1 ± 76.4 | 139.5 ± 6.9 | 1.1 ± 0.1 |
a
Wild-type strain.
b
Target gene for the expression analysis.
c
penR in B. cenocepacia J2315 is an ampR homolog.
d
ampD mutation in the strain.
e
Abbreviations for antibiotics used in the induction: AMX, amoxicillin; CAZ, ceftazidime; CTXM, cefotaxime; MER, meropenem.