TABLE 2.
Comparison between EUCAST and CLSI methods for antifungal susceptibility testing of Mucoralesd
| Comparison and species | No. of strains | % essential agreement, median (range) 2-fold difference |
% categorical agreement |
||||||
|---|---|---|---|---|---|---|---|---|---|
| AMB | POSA | ISA | VORI | AMB | POSAa | ISA | VORI | ||
| EUCAST day 1 vs CLSI | |||||||||
| Lichtheimia corymbifera | 12 | 100, 0 (−1 to 1) | 100, −0.5 (−1 to 0) | 100, 0 (−1 to 1) | 88, 0 (−2 to 0) | 100 | 100/100 | 83 | 100 |
| Lichtheimia ramosa | 4 | 100, 0 (0–0) | 75, −1 (−3 to 1) | 75, −1 (−3 to 1) | 75, 0.5 (0–2) | 100 | 100/100 | 100 | 100 |
| Mucor circinelloides | |||||||||
| Group I | 4 | 100, 0 (0–0) | 100, 0 (−1 to 0) | 75, 1 (0–2) | 0, 3b | 100 | 50/75 | 100 | 67 |
| Group II | 9 | 100, 0 (−1 to 1) | 43, 0 (−2 to 3) | 100, 0 (0–1) | 100, −1b | 100 | 100/67 | 100 | 100 |
| Rhizomucor pusillus | 8 | 100, 0 (0–0) | 63, −1 (−2 to 1) | 88, 0 (−1 to 2) | 88, −0.5 (−1 to 2) | 100 | 100/100 | 100 | 88 |
| Rhizopus microsporus | 26 | 73, 1 (−1 to 4) | 73, 1 (0–2) | 54, 1 (0–3) | 75, 1 (−1 to 3) | 100 | 12/100 | 92 | 100 |
| Rhizopus oryzae | 6 | 17, 2 (1–2) | 83, 0.5 (−1 to 2) | 67, 0.5 (0–2) | 100, 1 (0–1) | 100 | 50/83 | 67 | 100 |
| All | 70 | 83, 0 (−1 to 4) | 76, 0 (−3 to 3) | 75, 1 (−3 to 3) | 81, 0 (−2 to 3) | 100 | 59/93 | 91 | 97 |
| EUCAST day 2 vs CLSI | |||||||||
| Lichtheimia corymbifera | 12 | 58, 1 (0–2) | 100, 1 (−1 to 1) | 75, 1 (0–2) | NDc | 100 | 75/100 | 67 | 100 |
| Lichtheimia ramosa | 4 | 100, 1 (0–1) | 80, 0 (0–2) | 80, 1 (0–2) | 0, 3b | 100 | 40/100 | 80 | 100 |
| Mucor circinelloides | |||||||||
| Group I | 4 | 75, 1 (0–2) | 50, 2 (0–3) | 25, 2 (1–3) | ND | 100 | 25/75 | 100 | 67 |
| Group II | 9 | 89, 1 (0–2) | 50 (0 and 3b) | 67, 1 (1–2) | ND | 100 | 89/89 | 89 | 100 |
| Rhizomucor pusillus | 8 | 100, 1 (0–1) | 89, 1 (0–2) | 67, 1 (0–3) | 86, 1 (0–4) | 100 | 78/100 | 100 | 89 |
| Rhizopus microsporus | 26 | 35, 2 (0–5) | 16, 3 (1–5) | 4, 3 (1–6) | 33, 2 (0–3) | 100 | 0/35 | 35 | 100 |
| Rhizopus oryzae | 6 | 0, 3 (2–4) | 80, 1 (0–2) | 17, 2 (1–2) | 80, 1 (1–2) | 100 | 17/83 | 50 | 100 |
| All | 70 | 58, 1 (0–5) | 61, 1 (−1 to 5) | 38, 2 (0–6) | 59, 1 (0–4) | 100 | 39/72 | 63 | 97 |
a
Categorical agreement using the cutoffs of 0.25 mg/liter and 1 mg/liter.
b
Fewer than 3 isolates with on-scale MICs. The 2-fold dilution differences are presented for each isolate.
c
ND, not determined because of off-scale MICs.
d
Abbreviations: AMB, amphotericin B; POSA, posaconazole; ISA, isavuconazole; VORI, voriconazole.