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A. baumannii NaxD is required for polymyxin resistance. The A. baumannii wild-type strain, polymyxin-susceptible A. baumannii strain 17978 (WT), polymyxin-resistant strain R2, the R2ΔnaxD mutant, and the naxD-complemented strain (R2ΔnaxD comp) were incubated with 7 μg/ml polymyxin B or 10 μg/ml colistin for 2 h, and CFU were enumerated after plating. **, P < 0.005; ***, P < 0.0005.
