Figure 5.

mRNA expression of tumor necrosis factor (TNF)-α, IL-1β, cyclooxygenase 2 (COX-2), IL-6, IL-8, and matrix metalloproteinase 9 (MMP9) in monocyte-derived macrophages from healthy controls (n = 5). Cells were stimulated with lipopolysaccharide for 4 hours and pretreated with a RWJ 67657 at various concentrations. mRNA expression was determined with real-time RT-PCR and results are expressed as fold induction in comparison with unstimulated cells (fold induction = 1). Bars show means and SEM (*P < 0.05, paired t-test, calculated against the stimulated control).