TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Genotype/relevant characteristic(s)a | Reference or source |
|---|---|---|
| Strain | ||
| E. cloacae subsp. cloacae ATCC 13047 | Reference strain completely sequenced and annotated | 41 |
| ECLΔampC | 13047 derivative with deletion of ampC (ECL_00553) | This study |
| ECLΔampR | 13047 derivative with deletion of ampR (ECL_00554) | This study |
| ECLΔampD | 13047 derivative with deletion of ampD (ECL_00906) | This study |
| ECLΔampE | 13047 derivative with deletion of ampE (ECL_00907) | This study |
| ECLΔampG | 13047 derivative with deletion of ampG (ECL_01191) | This study |
| ECLΔampH | 13047 derivative with deletion of ampH (ECL_01132) | This study |
| ECLΔdacB | 13047 derivative with deletion of dacB (ECL_04561) | This study |
| ECLΔdacBΔampC | 13047 derivative with deletion of dacB and ampC | This study |
| ECLΔdacBΔampR | 13047 derivative with deletion of dacB and ampR | This study |
| ECLΔnagZ | 13047 derivative with deletion of nagZ (ECL_02529) | This study |
| ECLΔ03254 | 13047 derivative with deletion of ECL_03254 | This study |
| ECLΔ03253 | 13047 derivative with deletion of ECL_03253 | This study |
| Plasmid | ||
| pKOBEG | Recombination vector, phage λ recγβα operon under the control of the pBAD promoter, Cmr | 42 |
| pKD4 | Plasmid containing an FRT-flanked kanamycin cassette, Kanr | 43 |
| pCP20_Gm | FLP-mediated recombination vector, Genr | 44 |
a
Cmr, chloramphenicol resistant; Genr, gentamicin resistant; Kanr, kanamycin resistant.