FIG 3.
CLA inhibits ToxT binding to PtcpA DNA. (A) Autoradiograph of the results of MBP-ToxT binding reactions with PtcpA DNA. Lanes 1 to 7 include DMSO, and lanes 8 to 14 include 32 μM CLA in DMSO. The autoradiograph of EMSAs presented is representative of three or more independent experiments. (B) Binding curve using densitometry of the experiment whose results are shown in panel A. GraphPad software analysis of the Kd indicates that CLA induces a significant reduction in the binding affinity of ToxT for its cognate DNA. Kd values are shown in the inset, with significant difference between the best-fit values indicated by an asterisk: *, P < 0.0001.