Skip to main content
. 2013 Dec 1;26(6):366–377. doi: 10.1089/vim.2013.0016

FIG. 6.

FIG. 6.

Confirmation of TLR3 in JEV infection in Neuro2a cell line at 18 h post infection. (A) immunofluorescence assay for detection of JEV-E protein in JEV-infected and JEV-infected plus TLR3 gene silenced Neuro2a cells as compared to uninfected cells. Note that JEV-E protein was increased in TLR3 silenced JEV-infected Neuro2a as compared to JEV-infected Neuro2a cells. (B) represents the amplification plot of real-time PCR for JEV-NS5 gene. X-axis depicts RT-PCR cycles and Y-axis depicts delta Rn. Note that Ct value of JEV-infected Neuro2a cells was 25.5, while it was 16.67 in JEV-infected and TLR3 silenced Neuro2a cells, indicating enhanced virus production.