Table 3.

Real-Time PCR Results Obtained in JEV-Infected Neuro2a Cells With and Without TLR3 Gene Silencing

	Uninfected Neuro2a cells		JEV-infected Neuro2a cells*				JEV-infected TLR3 silenced Neuro2a cells*
Name of the gene	Mean-Ct	ΔCt	Mean-Ct	ΔCt	ΔΔCt	Fold change	Mean-Ct	ΔCt	ΔΔCt	Fold change
TLR3	24.12	2.44	22.03	0.16	−2.28	4.85	23	2.91	0.47	−1.38
GAPDH	21.56		21.87				20.09

^*JEV-NS5 gene was used to estimate viral RNA in infected Neuro2a cells. The mean Ct values for JEV-infected Neuro2a was 25.5 and for JEV-infected TLR3 silenced Neuro2a cells was 16.67.