Figure 1.

Generation of IdHP Cells from Murine HSCs or HPCs

(A) Schematic representation of IdHP cell generation.

(B) Flow cytometric analysis of control (empty vector) and ID3-overexpressing FL progenitor cells (n = 3).

(C) Photomicrograph of IdHP cells. Scale bar, 10 μm.

(D) Wright’s staining of IdHP cells, LKS cells, pro B cells, and immature B (Immat B) cells from BM. Scale bars, 10 μm.

(E) Microarray analysis of gene expression in LKS cells, E2A^−/− HPCs, IdHP cells, and pro B cells derived from cultures of control vector-expressing FL progenitors.

(F) In vitro expansion of IdHP and control cells. Viable cells were counted at an each time point (n = 3).

(G) CFU-C assay of LKS, control, and IdHP cells (n = 3).

(H) Myeloid and B cell generation from IdHP cells in vitro. Flow cytometric profiles of IdHP cells cultured on TSt-4 stromal cells for 14 days are shown (n = 3).

(I) T cell generation from IdHP cells in vitro. Flow cytometric profiles of control and IdHP cells cultured on TSt-4/DLL1 stromal cells for 12 days are shown (n = 3).

(J) The number of MAC1⁺ cells generated from IdHP and control cells on TSt-4 stromal cells is shown (n = 3). The FACS profile from IdHP cells is shown in (H).

(K) The number of CD4⁺CD8⁺ cells generated from IdHP cells on TSt-4/DLL1 stromal cells is shown (n = 3). The FACS profiles are shown in (H) and (I). Student’s t test, ^∗p < 0.05, ^∗∗∗p < 0.001. Data are shown as mean ± SD from three independent experiments.

See also Figure S1.