Figure 5.

Inducible Generation of IdHP Cells Using an ID3-ER Retrovirus

(A) Developmental potential of FL (LKS) progenitors transduced with control (ER) and ID3-ER retrovirus. After transduction, GFP⁺ cells were sorted and cultured on TSt-4 stromal cells supplemented with SCF, IL-7, and FLT3-L in the presence or absence of 4-OHT for 4 weeks. Representative flow cytometric profiles for CD19 versus B220 are shown.

(B) Expansion of FL (LKS) progenitors transduced with control and ID3-ER retrovirus in the presence of 4-OHT. Viable cells were counted at the indicated time points.

(C) Number of LIN⁻ cells from control and ID3-ER infected cells after 32 days of culture.

(D) B cell generation from ID3-ER-transduced cells after withdrawal of 4-OHT. The percentage of CD19⁺ cells at the indicated time points is shown. ^∗∗p < 0.01, ^∗∗∗p < 0.001. Data are shown as mean ± SD from three independent experiments.

See also Figure S5.