Figure 1.

BMP Treatment Reduces Proliferation of GNS and NS Cells

(A) Proliferation curves of seven GNS and two NS cells (NS-1 and NS-2). At day 7 all paired comparisons (GF versus BMP4) showed a significant difference in proliferation rate (p < 0.01).

(B) Cells were expanded in the GFs EGF and FGF-2 (GF) or exposed to BMP4 in the absence of GFs for 8 days (BMP). Proliferation was assessed by EdU (16 hr incorporation) and astrocyte differentiation using GFAP (red).

(C) Quantification of EdU-positive cells in proliferating conditions (GF), GF withdrawal (GF−), and BMP4 is shown.

(D) Immunostaining for cell-cycle marker MCM2 (red) and quantification (bottom) are shown.

(E) Relative mRNA expression levels of the BMPR1B in NS and GNS cell lines (fold change relative to normal brain). Error bars denote SD of two technical and two biological replicates for (C)–(E) (triplicates for immunostainings). Scale bars in (B) and (D), 100 μm.