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. 2015 Oct 22;5(5):829–842. doi: 10.1016/j.stemcr.2015.09.014

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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BMP-Treated Cells Do Not Undergo Terminal Differentiation

(A) G26 under self-renewal conditions (GF), treated with BMP4 for 7 (B7) and 54 days (B54), and when adding back GFs after the respective treatment times for 4 days (B7 + GF4 and B54 + GF4) is shown.

(B) Quantitation of EdU-positive cells from (A). Error bars denote SD of five technical replicates. Student’s two-sided t test was used for pairwise comparisons (^∗p ≤ 0.05).

(C) The qPCR analysis of G26 to determine the effects of re-exposure to GFs on astrocyte markers (AQP4 and GFAP) and NS cell-associated markers (EGFR, SOX2, and OLIG2). Error bars denote SD of two technical replicates from two biological replicates relative to cultures in GFs at day 0.

(D) Dendrogram shows the 450K methylation data in G26 before and after the readdition of EGF and FGF-2 for 4 days (GF).

(E) Kaplan-Meier curve showing survival data of mice transplanted with G26 grown under self-renewal conditions (GF), treated with BMP4 for 7 (B7) and 54 days (B54), and when adding back GFs after the respective treatment times for 4 days (B7 + GF4 and B54 + GF4) (n = 4–7 mice in each group). Scale bars, 100 μm.