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. 2015 Oct 29;5(5):816–828. doi: 10.1016/j.stemcr.2015.09.019

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© 2015 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Activation of SHH Pathway Enhances Symmetric Divisions in the Neonatal Cerebellum Ex Vivo

(A) A Math1-GFP;Dcx-DsRed;Patched^+/− mice strain was developed by crossing Math1-GFP;Dcx-DsRed mice and Patched^+/− mice. Patched1, a key factor of SHH pathway, was mutated in Patched^+/− mice, resulting in activation of hedgehog signaling. 3D images of the cerebella were captured from Math1-GFP;Dcx-DsRed;Patched^+/+ mice (a–d) or Math1-GFP;Dcx-DsRed;Patched^+/− (a′–d′) mice at P9, P13, P16, and P21, respectively. Scale bars, 100 μm.

(B) Percentages of MATH1-positive cells (green), MATH1 and DCX double-positive cells (yellow), and DCX-positive cells (red) were calculated and compared between the two mouse strains. The results indicate that the percentage of MATH1-positive cells (progenitor cells) over the total cells increased by 25.33%, 23.67%, 29.01%, and 26.35% in Patched mutant mice at P9, P13, P16, and P21, respectively, while the number of MATH1 and DCX double-positive cells (intermediate cells, yellow) plus sole DCX double-positive cells (red) decreased significantly in the Math1-GFP;Dcx-DsRed;Patched^+/− mice. M+D+P^(+/+), Math1-GFP;Dcx-DsRed;Patched^+/+ mice; M+D+P^(+/−), Math1-GFP;Dcx-DsRed;Patched^+/− mice.

The data were collected from three animals for Math1-GFP;Dcx-DsRed;Patched^+/+ mice and from five animals for Math1-GFP;Dcx-DsRed;Patched^+/− mice. Data are shown as mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001, referring to the number of MATH1-positive cells in Math1-GFP;Dcx-DsRed;Patched^+/+ versus Math1-GFP;Dcx-DsRed;Patched^+/− compound mice at P9, P13, P16, and P21.

See also Figure S2.