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. 2015 Nov 18;5:16763. doi: 10.1038/srep16763

Figure 2. Characterisation of purified MNs obtained from adult spinal cord.

Figure 2

MNs (with a 90% purity) were extracted from 5-month-old SOD1G93A mice at disease onset, and cultured for one day (A–D) and 15 days (E,F), and characterized for expression of CHAT (in green, (A,D,E)), Islet1 (in green, (B)) and NFM (in red, (B,F)), TDP-43 (in green, (C)) and NeuN (in red, (C)), TUJ1 (in red, (E,D), and in green, (F)) and NFM (in red, (F)) (200,000 cells/cm2). The yield of MNs obtained from different mouse lineages 1, 5, 11 and 12 months after birth was quantified and showed significant variations from 19 000 to 65 000 MNs (*p < 0.05) (G). Scale bars 20 μm.