Figure 6. Characterisation of small gamma MNs from adult spinal cord from 5-month-old wtSOD1mice.

(A) Identification of extracted small gamma MNs by immunofluorescence staining after 21 days of culture using CHAT in green and NFM in red (A), ERR3 in green and TUJ1 in red (B), P75 in green and S100b in red (C) (50,000 cells/cm²). The TUJ1-positive MNs did not express GFAP (in green, (D)), NeuN (in red, (E)), AQP4 (in green, (G)), OLIG2 (in green) and CNPase in red (F). Nuclei were stained in blue with DAPI (A,D,E,I). The TUJ1-positive MNs formed aligned nerve bundles after 3 months in culture (H,I). Scale bar 20 μm.