Table 2. Effect of ion binding on EF-hands in PEF proteins.
| Prot (pdb) | EF1 (θ) [A-B] | EF2 (θ) [C-D] | EF3 (θ) [D-E] | EF4 (θ) [F-G] | EF5 (θ) [G-H] | D-helix vs G-helix (θ) |
|---|---|---|---|---|---|---|
| θ | θ | θ | θ | θ | ||
| hSorcin | ||||||
| ApoSor (4UPG) | 40.8° | 60.7° | 52° | 47.3° | 32.9° | 57.5° |
| CaSor (4USL) | 59.2° | 57.9° | 66.5° | 45.9° | 29.9° | 78.1° |
| +Ca | +Ca | +Ca | — | — | ||
| Δ (+Ca) | +18,4° | −2.9° | +14.5° | −1.40° | −3° | +20.6° |
| hPDCD6 | ||||||
| PDCD6-apo (2ZND) | 68.8° | 51.5° | 59.2° | 56.5° | 35.8° | 80.2° |
| PDCD6+Ca (2ZN9) A = B | 72° | 52.6° | 64° | 58.4° | 27.2° | 82.2° |
| +Ca | — | +Ca | — | +Ca | ||
| +3.2° | +1.1° | +4.8° | +2.1° | −8.6° | +2° | |
| hPDCD6-Zn-Alix(2ZNE) | 67.2 | 55.4 | 62.4 | 60.5 | A = 31.7 B = 36.6 | A = 74.5 B = 75.4 |
| +Zn | — | +Zn x2 | — | +Zn | ||
| Δ(+Zn, +pep) | −1.5° | +4° | +3° | +4° | A = −4° | A = −5.7° |
| B = +1° | B = −4.8° | |||||
| rCalpain-dVI | ||||||
| apo monA (1AJ5) | 40.3° | 53.6° | 30° | 51.5° | 28° | 47° |
| apo monB (1AJ5) | 41.2° | 52° (54°)* | 33.6° (28.5°)* | 44.5° | 20.5° | 48.6° |
| + Ca (1DVI) | 61° | 63° (71°)* | 46.2° (42°)* | 49.4° | 23.4° | 52.3° |
| +Ca | +Ca | +Ca | — | — | ||
| Δ (+Ca) | +20.7° | +9° | +16° | +1.6° | −3° | +5.3° |
| +19.7° | +11° | +12° | +3° | +4° | +3.7° | |
The angle between the helices has been calculated with PyMol. The angle between helices D and G is reported (θ), as an indicator of the movement of sub-domain EF1-EF2-EF3 with respect to EF4-EF5.