Figure 1.
CDKN2D expression is significantly lower in APL cells than that in normal promyelocytes. (a) The expression level of CDKN2D in APL patient samples was significantly lower than that in normal promyelocytes (Pro) and peripheral mononuclear cells (PMNs). The expression data were retrieved from microarray profiling performed by Payton JE et al. (GSE12662).18 The expression values are absolute intensities after being log-transformed. (b) The upper panel shows the induced expression of PML/RARα by ZnSO4 (Zn) in PR9 cells using western blot analysis. The lower panel shows the reduced expression of CDKN2D after the induction of PML/RARα. PR9 cells were treated with or without 100 μM ZnSO4 (Zn) for 4 h. The relative mRNA level of CDKN2D was normalized to GAPDH. * indicates P<0.05, *** indicates P<0.001