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. 2014 Oct 2;5(10):e1443. doi: 10.1038/cddis.2014.395

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/

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GBM cells with mesenchymal features have enhanced migratory capacity in vitro. (a) Phase contrast microscopic pictures ( × 10) of the newly generated GBM cell line GG7 along with the commercially available U87 and U251 cells. (b) Immunofluorescence analysis for mesenchymal (Fibronectin, COL5A1), proneural (PDGFR-α) and classic (EGFR) markers. (c) Wound healing assays comparing the migratory capacity of U87 versus GG7 cells at different time points. A representative experiment is shown. (d) Quantified data of wound healing assays where each data point represents the mean of at least three independent experiments±S.E.M. (***P<0.001 GG7 versus U87 for their respective time points)