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. 2014 Oct 9;5(10):e1452. doi: 10.1038/cddis.2014.403

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/

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ATF4 activates the JNK pathway. (a, left) Anti-activated caspase 3 (green) and anti-MMP1 (red) staining in vg-GAL4, UAS-Psn, UAS-Psn/+ (top) and vg-GAL4, UAS-Psn, UAS-Psn/+ UAS-diap1/+ (bottom) third-instar wing imaginal discs. (a, right) Intensity of GFP in vg-GAL4, UAS-Psn, UAS-Psn/+ dilp8^MI00727/+ (top) and vg-GAL4, UAS-Psn, UAS-Psn/+ dilp8^MI00727/UAS-diap1 (bottom) third-instar wing imaginal discs. (b) Quantification of relative dilp8::EGFP expression. Error bars represent the S.E.M. of at least nine independent experiments. The asterisk indicates significant difference between the indicated genotype and the control (P<10⁻⁵, ANOVA). (c) Anti-MMP1 staining (left) and intensity of Dilp8::EGFP detection (right) in vg-GAL4, UAS-Psn, UAS-Psn/+ (top) and vg-GAL4, UAS-Psn, UAS-Psn/+ UAS-atf4-RNAi (bottom) third-instar wing imaginal discs. (d) Quantification of relative dilp8 expression. Error bars represent the S.E.M. of at least four independent experiments. The asterisk indicates significant difference between the indicated genotype and the control (P<10⁻⁴, ANOVA). (e) Effects of ATF4 depletion (gray bars) compared with control (white bars) on vg-GAL4, UAS-Psn, UAS-Psn/+ (solid bars) and vg-GAL4/+ (streaked bars) on the mean time of adult eclosion. Error bars represent the S.E.M. (n=3). The asterisk corresponds to a statistical difference (P<10⁻³, ANOVA)