Figure 7.

DNA-damaging agent Dox induces expression of OXPHOS proteins and enhances activities of OXPHOS complex. (a) Mitochondria isolated from untreated and Dox (10 μM for 12 and 24 h)-treated HCT116 WT cells were subjected to western blotting for OXPHOS proteins. (b–f) Mitochondria isolated from HCT116 WT cells treated with Dox (10 μM) for 0, 12, and 24 h were lysed by three freeze-thaw cycles in hypotonic buffer. Equal amount of mitochondria were assayed to determine enzymatic rate of respiratory complexes. Specificity of all enzymatic rates was controlled by using specific enzyme inhibitors or negative controls in parallel reactions. (b–f) The fold change of specific activity of OXPHOS complexes (nmol/min/mg mitochondrial proteins). Data are mean±S.D., n=3, *P<0.05 and **P<0.01 compared DMSO-treated cells. Complex V-ATP5A serves as loading control. Dox, doxorubicin; Mito, mitochondria; HCT, HCT116