Figure 5.

SIRT1 is essential for the inhibition of LPS-induced HMGB1 release by PPAR-δ and -γ. (a and b) RAW 264.7 cells pretreated with resveratrol (a) or sirtinol (b) for 1 h were stimulated with LPS in the presence or absence of GW501516. (c and d) Cells pretreated with resveratrol (c) or sirtinol (d) for 1 h were incubated with LPS in the presence or absence of rosiglitazone. After incubation for 24 h, equal volumes of conditioned media were analyzed by western blotting with an anti-HMGB1 antibody. An image analyzer was used to quantitate band intensity, and the ratios of HMGB1 to Ponceau S are shown. The results are expressed as means±S.E.M. (n=3). **P<0.01 compared with the untreated group; ^##P<0.01 compared with the LPS-treated group; ^††P<0.01 compared with the group treated with LPS+GW501516 or rosiglitazone. Ponceau S staining was used as a loading control