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. 2014 Oct 2;5(10):e1436. doi: 10.1038/cddis.2014.414

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International Licence. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons licence, users will need to obtain permission from the licence holder to reproduce the material. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/

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Tregs enhance ESC proliferation and invasiveness. (a) ESCs from ectopic lesions from patients with endometriosis were co-cultured with or without CD4⁺CD25⁺Tregs for 48 h. BrdU proliferation (a), Matrigel invasion (b) and In-cell Western assays (c, d) were performed to examine the proliferation, invasiveness and MMP2/9, TIMP1/2, Cox-2 and p53 expression in ESCs, respectively. MMP2, MMP9, TIMP1 and TIMP1 (red), actin (green); Cox-2 and p53 (green), actin (red). Original magnification: × 200 (b). All data are expressed as the mean±S.D. *P<0.05 compared with the vehicle control