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. 2014 Oct 2;5(10):e1436. doi: 10.1038/cddis.2014.414

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International Licence. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons licence, users will need to obtain permission from the licence holder to reproduce the material. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/

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Tregs stimulation of ESC proliferation and invasiveness is dependent on IL-10 and TGF-β. (a) We treated ESCs isolated from eutopic endometrium from patients with endometriosis with rhIL-10 and/or rhTGF-β for 48 h and analyzed the proliferation (a), invasion (b) and the expression of MMP2, TIMP1 and Cox-2 (c). In addition, ESCs were co-cultured with CD4⁺CD25⁺ Tregs and/or anti-human IL-10, TGF-β, CD28, CTLA-4, CD39, CD73 neutralizing antibodies for 48 h. BrdU proliferation (d), Matrigel invasion (e) and In-cell Western assays (f) were performed to examine the proliferation, invasiveness, and MMP2, TIMP1 and Cox-2 expression in ESCs, respectively. MMP2 and TIMP1 (red), actin (green); Cox-2 (green), actin (red). All data are expressed as the mean±S.D.