Figure 7.

Knockdown of XIAP and EGFR expression in PC-9 cells. Cells were transfected with 50 nM XIAP siRNA, EGFR siRNA or negative control siRNA for 24 h and then incubated with 2 μM HM90822B for 24 h. (a) Cell growth was determined by counting trypan-blue unstained cells. (b) The expression levels of EGFR, XIAP, phosphor-p38, p38, phosphor-AKT, AKT and β-actin were determined by western blotting analysis. (c) Working model was depicted for cell growth-inhibitory effect of HM90822B in NSCLC cells