Figure 7.

MALAT1 knockdown prevents the hyper-proliferation of retinal endothelial cells through p38 MAPK signaling. (a and b) RF/6A cells were transfected with MALAT1 siRNA, scramble siRNA (Scr), or left untreated and then exposed to high glucose for 48 h. The untreated group was taken as the control group. Representative immunoblots of total p38, total ERK, total JNK, p-p38, p-ERK, p-JNK, and tubulin (a) are shown along with the densitometric quantitative results (b). (c) RF/6A cells were transfected with MALAT1 to promote cell viability and treated with SB203580, U0126, or SP600125 for 48 h. Cell viability was detected using the MTT method (n=4). (d) RF/6A cells were co-transfected with MALAT1 and treated with p38 siRNA, ERK siRNA, or JNK siRNA for 48 h. The cell viability was detected using the MTT assay (n=4). All data are shown as mean±S.E.M. *Indicates a significant difference compared with the corresponding control group