Figure 2.

Differential microRNA expression in adults and neonates. (A) PCA using RPM values of well-expressed miRNAs. Adult and neonatal naive samples consist of two highly correlated pooled biological replicates (Figure S2); naive newborn and 5- and 7-dpi samples each consist of one replicate. The percentage of the overall variation accounted for by principal components 1 (x-axis) and 2 (y-axis) is indicated for each axis. (B) Fold-change difference in expression between adults and neonates was found for miRNA families in naive and 5- and 7-dpi CD8+ T cells, as well as between naive adult and newborn cells. Reads for miRNAs corresponding to the same family were summed. Significance was determined by edgeR exact test with multiple test correction (Benjamini–Hochberg; *P < 0.05, **P < 0.005, ***P < 0.0005). Small RNA expression data were replicated with independent biological samples, using an alternative method of sequencing library construction (see Figure S2).