Skip to main content
. 2015 Sep 24;201(3):1031–1045. doi: 10.1534/genetics.115.180794

Figure 3.

Figure 3

Effects of a subset of histone mutations after integration into both genomic copies of the genes. The mutations shown were integrated into HTA1 and HTA2 or into HTB1 and HTB2 along with a genetic marker downstream of the ORF, as indicated previously (McCullough et al. 2011). Standard genetic crosses were performed to combine the histone mutations with one another and with the spt6 alleles indicated. The nomenclature used is “H2A-N39K” indicates a hta1-N39K hta2-N39K double mutant. (A) The effects of combining spt6-F249K with a subset of the histone mutations. (B) The effects of combining the spt6-F249K allele with normal and N39K-mutated versions (genetically marked in both cases) of HTA1 and HTA2. (C) The effects of replacing H2A-N39 with a basic, neutral, or acidic residue.