Skip to main content
. 2015 Sep 24;201(3):1031–1045. doi: 10.1534/genetics.115.180794

Table 1. Strains used.

Figure 1 W303 background
8857-13-1 MATa ade2 can1 his3 leu2 lys2-128∂ trp1 ura3 spt6-F249K hht1-hhf1-∆(::HIS3) hht2-hhf2-∆(::KanMX) hta1-htb1-∆(::NatMX) hta2-htb2-∆(::HphMX) pTF237 (YCp URA3 HHT2-HHF2, HTA1-HTB1)
Figure S1 W303 background
9029-3-2 MATa ade2 can1 his3 leu2 lys2-128∂ met15 trp1 ura3 spt16-11 hht1-hhf1-∆(::HIS3) hht2-hhf2-∆(::KanMX3) hta1-htb1-∆(::NatMX) hta2-htb2-∆(::HphMX) pTF237 (YCp URA3 HHT2-HHF2, HTA1-HTB1)
9028-6-1 MATa ade2 can1 his3 leu2 lys2-128∂ trp1 ura3 hht1-hhf1-∆(::HIS3) hht2-hhf2-∆(::KanMX3) hta1-htb1-∆(::NatMX) hta2-htb2-∆(::HphMX) pTF237 (YCp URA3 HHT2-HHF2, HTA1-HTB1)
9028-1-4 MATa ade2 can1 his3 leu2 lys2-128∂ trp1 ura3 pob3-Q308K hht1-hhf1-∆(::HIS3) hht2-hhf2-∆(::KanMX3) hta1-htb1-∆(::NatMX) hta2-htb2-∆(::HphMX) pTF237 (YCp URA3 HHT2-HHF2, HTA1-HTB1)
Figure 3A A364a background
8127-7-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂
9506-1-3 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, KanMX)
9008-2-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N39K(+220, His3MX) hta2-N39K(+30, URA3)
9022-6-2 MATα ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, KanMX) hta1-N39K(+220, His3MX) hta2-N39K(+30, URA3)
9021-2-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N100D(+220, His3MX) hta2-N100D(+30, URA3)
9034-3-1 MATα ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, KanMX) hta1-N100D(+220, His3MX) hta2-N100D(+30, URA3)
8523-2-1 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-V101I(+220, His3MX) hta2-V101I(+30, URA3)
9636-9-3 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-V101I(+220, His3MX) hta2-V101I(+30, URA3) spt6-F249K(-424, KanMX)
9019-1-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ htb1-L83F(+30, URA3) htb2-L83F(+30, His3MX)
9030-5-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ htb1-L83F(+30, URA3) htb2-L83F(+30, His3MX) spt6-F249K(-424, KanMX)
8984-L MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ htb1-A84D(+30, LEU2) htb2-A84D(+30, His3MX)
9487-1-3 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ htb1-A84D(+30, LEU2) htb2-A84D(+30, His3MX) spt6-F249K(-424, URA3)
Figure 3B A364a background
8127-7-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂
9659-10-3 MATα ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, KanMX)
9659-6-4 MATα ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, KanMX) hta1-N39K(+220, His3MX)
9659-7-3 MATα ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, KanMX) hta2-N39K(+30, URA3)
9659-7-4 MATα ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, KanMX) hta1-N39K(+220, His3MX) hta2-N39K(+30, URA3)
Figure 3C A364a background
8127-7-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂
9506-1-3 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, KanMX)
9008-2-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N39K(+220, His3MX) hta2-N39K(+30, URA3)
9659-8-3 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, KanMX) hta1-N39K(+220, His3MX) hta2-N39K(+30, URA3)
9705-1-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N39A(+220, His3MX) hta2-N39A(+30, URA3)
9712-8-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N39A(+220, His3MX) hta2-N39A(+30, URA3) spt6-F249K(-424, KanMX)
9709-5-1 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N39D(+220, His3MX) hta2-N39D(+30, URA3)
9713-1-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N39D(+220, His3MX) hta2-N39D(+30, URA3) spt6-F249K(-424, KanMX)
Figure 4A Strains for Spt6 abundance measurement (S288c background)
8205-3-1 MATa ura3-52 leu2-∆1 trp1-∆63 his4-912∂ lys2-128∂
8142-8-1 MATa ura3-52 leu2-∆1 his4-912∂ lys2 spt6-14
8164-3-3 MATa ura3-52 leu2-∆1 trp1-∆63 his4-912∂ lys2-128∂ spt6-50
8205-5-3 MATa ura3-52 leu2-∆1 trp1-∆63 his4-912∂ lys2-128∂ FLAG-spt6-1004
8789-1-3 MATa ura3-52 leu2-∆1 trp1-∆63 his4-912∂ lys2-128∂ spt6-F249K(-424, URA3)
Figure 4B Strains for histone abundance measurement (A364a background)
8500-10-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ HTA1(+220, His3MX) HTA2(+30, URA3)
8483-9-1 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ HTB1(+30, URA3) HTB2(+30, His3MX)
9008-2-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N39K(+220, His3MX) hta2-N39K(+30, URA3)
9021-2-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N100D(+220, His3MX) hta2-N100D(+30, URA3)
9019-1-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ htb1-L83F(+30, URA3) htb2-L83F(+30, His3MX)
8442-4-3 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ htb1-A84D(+30, URA3) htb2-A84D(+30, His3MX)
9430-2-1 ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta2-htb2-∆(::HphMX)
9506-1-3 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, KanMX)
Figure 4C Diploids (A364a background)
8274 MATa/MATα ura3-∆0/ura3-∆0 leu2-∆0/leu2-∆0 trp1-∆2/trp1-∆2 his3/+ +/his7 lys2-128∂/lys2-128∂
8537-1 MATa/MATα ura3-∆0/ura3-∆0 leu2-∆0/leu2-∆0 trp1-∆2/trp1-∆2 his3/+ +/his7 lys2-128∂/lys2-128∂ spt6-∆(::KanMX)/SPT6
9465 MATa/MATα ura3-∆0/ura3-∆0 leu2-∆0/leu2-∆0 trp1-∆2/trp1-∆2 his3/+ +/his7 lys2-128∂/lys2-128∂ spt6-F249K(URA3, -424)/SPT6
9562 MATa/MATα ura3-∆0/ura3-∆0 leu2-∆0/leu2-∆0 trp1-∆2/trp1-∆2 his3/+ +/his7 lys2-128∂/lys2-128∂ spt6-F249K(-424, URA3)/spt6-F249K(-424, URA3)
Figure 4D Diploids (A364a background)
8274 MATa/MATα ura3-∆0/ura3-∆0 leu2-∆0/leu2-∆0 trp1-∆2/trp1-∆2 his3/+ +/his7 lys2-128∂/lys2-128∂
9582 MATa/MATα ura3-∆0/ura3-∆0 leu2-∆0/leu2-∆0 trp1-∆2/trp1-∆2 his3/+ +/his7 lys2-128∂/lys2-128∂ spt6-∆(::KanMX)/SPT6
9191 MATa/MATα ura3-∆0/ura3 leu2-∆0/leu2 trp1-∆2/trp1 his3/+ +/his7 lys2-128∂/lys2-128∂ pob3-∆(::LEU2)/POB3
9649 MATa/MATα ura3/ura3 leu2/leu2 trp1/trp1 his3/+ +/his7 lys2-128∂/lys2-128∂ spt6-∆(::KanMX)/SPT6 pob3-∆2(::LEU2)/POB3
9650 MATa/MATα ura3-∆0/ura3 leu2-∆0/leu2 trp1-∆2/trp1 his3/his3 lys2-128∂/lys2-128∂ spt16-∆(::HIS3)/SPT16
9629 MATa/MATα ura3-∆0/ura3 leu2-∆0/leu2 trp1-∆2/trp1 his3/his3 lys2-128∂/lys2-128∂ spt6-∆(::KanMX)/SPT6 spt16-∆(::HIS3)/SPT16
Figure 5A A364a background
8127-7-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂
8824-1-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spn1-F267E(+49, TRP1)
9008-2-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N39K(+220, His3MX) hta2-N39K(+30, URA3)
9025-4-1 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N39K(+220, His3MX) hta2-N39K(+30, URA3) spn1-F267E(+49, TRP1)
9021-2-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N100D(+220, His3MX) hta2-N100D(+30, URA3)
9037-2-1 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N100D(+220, His3MX) hta2-N100D(+30, URA3) spn1-F267E(+49, TRP1)
9019-1-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ htb1-L83F(+30, URA3) htb2-L83F(+30, His3MX)
9033-2-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ htb1-L83F(+30, URA3) htb2-L83F(+30, His3MX) spn1-F267E(+49, TRP1)
Figure 5B A364a background (V5 epitopes were added after Spt6 A1247 or in place of the native stop codon as a control for the tagged truncation.)
8127-7-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂
9008-2-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta1-N39K(+220, His3MX) hta2-N39K(+30, URA3)
9565-8-1 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, HphMX)
9659-8-3 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, KanMX) hta1-N39K(+220, His3MX) hta2-N39K(+30, URA3)
9755-1-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-A1247-V5(KanMX)
9775-5-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-A1247-V5(KanMX) hta1-N39K(220, His3MX) hta2-N39K(30, URA3)
9756-1-2 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ SPT6-V5(KanMX)
9776-2-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ SPT6-V5(KanMX) hta1-N39K(220, His3MX) hta2-N39K(30, URA3)
Figure S2 A364a background
8127-7-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂
9430-2-4 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta2-∆-htb2-∆(::HphMX)
9560-1-3 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ spt6-F249K(-424, URA3)
9587-4-3 MATa ura3-∆0 leu2-∆0 trp1-∆2 his3 lys2-128∂ hta2-∆-htb2-∆(::HphMX) spt6-F249K(-424, URA3)
Figure S3 S288c background
8266-7-5a leu2-∆1 trp1-∆63 ura3-52 his4-912∂ lys2-128∂
9710-1-4 ura3-52 leu2-∆1 trp1-∆63 his4-912∂ lys2-128∂ hta1-N39K(+220, NatMX) hta2-N39K(+30, URA3)
9584-1-2 ura3 leu2 trp1 his4-912∂ lys2-128∂ FLAG-spt6-1004
9717-5-2 ura3 leu2 trp1 his4-912∂ lys2-128∂ FLAG-spt6-1004 hta1-N39K(+220, NatMX) hta2-N39K(+30, URA3)
9583-8-1 ura3 leu2 trp1 his4-912∂ lys2-128∂ spt6-14
9714-3-4 ura3 leu2 trp1 his4-912∂ lys2-128∂ spt6-14 hta1-N39K(+220, NatMX) hta2-N39K(+30, URA3)
9571-2-4 ura3 leu2 trp1 his4-912∂ lys2-128∂ spt6-50
9715-4-3 ura3 leu2 trp1-∆63 his4-912∂ lys2-128∂ spt6-50 hta1-N39K(+220, NatMX) hta2-N39K(+30, URA3)

For Figure 1 and Figure S1A–C, the strain listed was used as the parent for the plasmid swaps that replaced pTF237 (YCp URA3 HHT2-HHF2, HTA1-HTB1) with the similar plasmids marked with LEU2 that carry the histone gene mutations noted in each case.