Skip to main content
. 2015 Sep 24;201(3):1031–1045. doi: 10.1534/genetics.115.180794

Table 3. Effect of temperature shift on Spt6 stability in Ts mutants.

Spt6 allele Signal at 30° Signal after 3 hr at 37° Retention at 37°
WT Spt6 100% 96 ± 6.6% 96%
Spt6-14 82 ± 32% 21 ± 12% 25%
Spt6-50 77 ± 15% 97 ± 24% 125%
Spt6-1004 40 ± 27% 24 ± 1.1% 60%
Spt6-F249K 71 ± 30% 81 ± 11% 115%

The same strains as in Figure 4A were tested under the conditions listed; signals were normalized to Spt6 WT at 30°; experiments were performed in triplicate with the standard error shown.