Table 3. Effect of temperature shift on Spt6 stability in Ts− mutants.
Spt6 allele | Signal at 30° | Signal after 3 hr at 37° | Retention at 37° |
---|---|---|---|
WT Spt6 | 100% | 96 ± 6.6% | 96% |
Spt6-14 | 82 ± 32% | 21 ± 12% | 25% |
Spt6-50 | 77 ± 15% | 97 ± 24% | 125% |
Spt6-1004 | 40 ± 27% | 24 ± 1.1% | 60% |
Spt6-F249K | 71 ± 30% | 81 ± 11% | 115% |
The same strains as in Figure 4A were tested under the conditions listed; signals were normalized to Spt6 WT at 30°; experiments were performed in triplicate with the standard error shown.