Figure 6.

Expression of the spe-47:GFP transcriptional reporter. Here, the worms expressed an integrated GFP flanked by the spe-46 promoter and 3′ UTR, and the tissue was labeled with the DNA dye Hoechst 33342. (A) In male gonads, GFP expression begins close to the end of the pachytene phase. (B) In gonads dissected from L4 hermaphrodites, GFP expression is evident in spermatogenic tissue at the same developmental stage as it is in the male gonad. Spermatogenesis proceeds from left to right in the regions of the gonads expressing GFP. Both bars, 25 µm.