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. 2015 Sep 8;201(3):885–895. doi: 10.1534/genetics.115.180653

Figure 6.

Figure 6

Establishment of sensitivity to SAC knockdown in FBW7 knockout cells requires multiple substrates. (A) Percentages of G1-, S-, and G2/M-phase cells in HCT116 cells overexpressing nondegradable forms of cyclin E and MCL1. (B) Western blot of HCT116 colorectal cell lines overexpressing nondegradable forms of MCL1 and full-length cyclin E or cyclin E lacking the N-terminal 65 residues (Δ65 cyclin E). (C) Percentages of >4 N cells in three independent experiments. (D) Percentages of cells in the sub-G1 range in three independent experiments. (E) HCT116 cells show sensitivity to BUBR1 knockdown only after expression of MCL1 and the Δ65 form of cyclin E. (*) P < 0.05; (**) P < 0.005; (***) P < 0.0005.