Figure 11.

Nuclear Gln3-Myc¹³ export of wild-type Gln3-Myc¹³ (pRR536, panel A), 64drdd Gln3-Myc¹³ (pRR536, Panel B), and 332dddd Gln3-Myc¹³ (pRR787, Panel C) in cells provided with a range of repressive to moderately repressive nitrogen sources. Cells were grown to mid-log phase in YNB medium containing the indicated nitrogen sources (asparagine, Asn; glutamine, Gln; glutamate + ammonia, Glt + Am; ammonia, Am; glutamate, Glt; or serine, Ser) before being sampled for assay of intracellular Gln3-Myc¹³ localization as in Figure 1, C–E.