Table 1. Strains used in this work.
| Strain | Pertinent genotype | Complete genotype |
|---|---|---|
| JK9-3daa | Wild type | MATa, leu2-3,112, ura3-52, trp1, his4, rme1, HMLa |
| TB50b | Wild type | MATa, leu2-3, 112, ura3-52, trp1, his3, rme1, HMLa |
| TB123b | Wild-type Gln3-Myc13 | MATa, leu2-3, 112, ura3-52, trp1, his4, rme1, HMLa, GLN3-MYC13[KanMX] |
| FV007 | gln3Δ, gat1Δ | (MATa, gat1Δ::natMX, gln3Δ::kanMX, leu2-3,112, ura3-52, trp1, his4, rme1, HMLa) |
| RR215 | ure2Δ | MATa, leu2-3,112, ura3-52, trp1, his4, rme1, HMLa,ure2Δ::kanMX |
a
JK9-3d was constructed by Jeanette Kunz (Michael Hall’s laboratory). Joseph Heitman isolated MATa and MATα strains isogenic to JK9-3d by mating-type switching. JK9-3da is a hybrid strain containing contributions from the following strains: S288c, a strain from the Oshima Laboratory, and an unidentified strain from the Herskowitz Laboratory. It was chosen because of its robust growth, sporulation efficiency, and good growth on galactose (GAL+). It may have a SUP mutation that allows translation through premature STOP codons and therefore produces functional alleles with many point mutations.
b
JK9-3da is the parent of TB50, and TB50 is the parent of TB123.