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. 2015 Mar 6;5:8816. doi: 10.1038/srep08816

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(A–B). mRNA expression analysis of insulin (INS) in human primary subcutaneous and visceral preadipocytes and adipocytes. Relative Insulin mRNA levels expressed as²log in (Panel A) and as relative intensity in (panel B). mRNA chip (human gene chip) approach was used to detect insulin mRNA expression. Each sample was measured 4 times by mRNA expression assay (human gene chip). (Panel C). mRNA expression of insulin and glucagon in human pancreas and adipose tissue (AT) with and without type 2 diabetes (T2D). The mRNA expression levels of insulin and almost glucagon remained unchanged in pancreas of either state. The mRNA expression of insulin was decreased and glucagon demolished in AT-T2D as compared to AT without T2D.