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. 2015 Nov 18;5:16748. doi: 10.1038/srep16748

Figure 4. Food-related cues induce circadian rhythmicity in ghrelin release by MGN3-1 ghrelinoma cells.

Figure 4

(a) Octanoyl ghrelin release, (b) total ghrelin release and relative mRNA expression levels of (c) ghrelin and (d) GOAT of serum shocked MGN3-1 cells after a 3-hour stimulation with vehicle (HEPES), peptone (3%) or L-epinephrine (5 μM) at 4-hour intervals (N = 3 independent experiments; n = 14 wells/stimulus/time point). (e) Octanoyl ghrelin release and (f) total ghrelin release of MGN3-1 cells transfected with Bmal1 siRNA or a non-targeting siRNA pool as negative control, after a 3-hour stimulation with vehicle (HEPES), peptone (3%) or L-epinephrine (5 μM) (N = 2 independent experiments; n = 8 wells/stimulus). *P < 0.001 vs. HEPES. (AU = arbitrary unit).