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. 2015 Nov 18;5:16747. doi: 10.1038/srep16747

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Copyright © 2015, Macmillan Publishers Limited

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(A) Orai1/Stim1 currents elicited by a ramp protocol ranging from −100 to +100 mV at pH_o 7.4, 5.5 and 8.2. Note the significant increase by pH_o 8.2 and marked inhibition by pH_o 5.5. (B) Concentration-dependent effects of pH_o on Orai1/STIM1. Inward current was measured at −100 mV. The effects of pH_o was evaluated by perfusing the cells with different pH_o in DVF after activation of Orai1/STIM1 in pH_o 7.4 DVF, and NMDG solution was perfused to ensure that there was no leak current during the experiment. (C) Changes of current amplitude at each pH_o normalized to the current amplitude at pH_o 7.4. The best fit of the dose-response curve yielded pKa of 8.26 ± 0.11 (n = 8). (D) Dose-response curve analyzed by normalizing current amplitude at each pH_o to the maximal current amplitude. Best fit of the dose-response curve produced similar pKa (8.32 ± 0.11, n = 8) to that shown in (C).