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. 2015 Nov 18;5:16747. doi: 10.1038/srep16747

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(A) Representative Orai1/Stm1 currents elicited by a ramp protocol ranging from −100 to +100 mV in cells with pipette solution at pH_i 5.5, 7.4 and 8.4, respectively. (B) Concentration-dependent effects of pH_i on Orai1/STIM1 currents. Current amplitude measured at −100 mV at each pH_i was normalized to the current amplitude at pH_i 7.4. Current amplitude was almost completely inhibited at pH_i 5, and was enhanced by about 2-fold at pH_i 9 (n = 12, 5, 6, 44, and 6 at pH_i 5.5, 6.5, 7.4, 8.4, and 9, respectively). (C) Dose-response curve analyzed by normalizing current amplitude at each pH_i to the maximal current amplitude at pH_i 9. Best fit of the dose-response curve produced same pKa (7.46 ± 0.18, n = 12, 5, 6, 44 at pH_i 5.5, 6.5, 7.4, 8.4, and 9, respectively).