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. 2015 Nov 18;5:16747. doi: 10.1038/srep16747

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Copyright © 2015, Macmillan Publishers Limited

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(A) Representative recording of I_CRAC in RBL cells. Currents were elicited by a ramp protocol ranging from −100 to +100 mV in DVF solutions. After I_CRAC activation reached a steady-state, the cell was exposed to external solutions with different pH. (B) Concentration-dependent effects of pH_o on I_CRAC. NMDG was used to test leak current. The inhibition and potentiation effects of pH_o were reversible. (C) Dose-response curve constructed using current normalized to the current amplitude at pH_o 7.4. The best fit of the dose-response curve yielded pKa of 8.41 ± 0.10 (Mean ± SEM, n = 8–11). (D) Representative traces of I_CRAC at pH_i 5.5, 7.4, and 8.5. (E) Average current amplitude of I_CRAC at pH_i 5.5, 7.4 and 8.5. (F) Dose-response curve constructed using current normalized to the current amplitude at pH_i 7.4. The best fit of the dose-response curve yielded pKa of 7.82 ± 0.2 (Mean ± SEM, n = 7–13).