Figure 4.

JWA enhances cisplatin-induced cell death through the regulation of DNA damage-induced apoptosis in gastric cancer cells. (a) The BGC823 and SGC7901 cells transfected with JWA siRNA for 48 h followed by exposure to 0.8 μg/ml of cisplatin for additional 12 h, and western blotting was used to determine the expression of JWA, γH2AX and PARP-1. (b) The BGC823 and SGC7901 cells were transfected with flag-JWA for 48 h and followed by exposure to 0.8 μg/ml of cisplatin for additional 4 and 8 h, and western blotting was used to determine the expression of flag-JWA, γH2AX and PARP-1