Figure 6.

Rescued JWA expression in cisplatin-resistant gastric cancer cells causes more sensitive to cisplatin-induced apoptosis and cell death. (a) The BGC823/DDP and SGC7901/DDP cells were transfected with flag-JWA for 48 h and followed by exposure to 10 μg/ml of cisplatin for 48 h, and the apoptotic rate was determined by the TUNEL assay ( × 100). (b) Quantification of TUNEL-positive BGC823/DDP and SGC7901/DDP cells transfected with flag-JWA. (c) The BGC823/DDP and SGC7901/DDP cells were transfected with flag-JWA for 48 h and subjected to clonogenic survival assay 3 weeks after treatment with cisplatin for 2 h. (d) Quantify numbers of colony in BGC823/DDP and SGC7901/DDP cells transfected with flag-JWA, each colony containing cells >50 were counted. *P<0.05, **P<0.01