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. 2014 Dec 11;5(12):e1571. doi: 10.1038/cddis.2014.540

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Copyright © 2014 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International Licence. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons licence, users will need to obtain permission from the licence holder to reproduce the material. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0

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Knockdown of BMPR2 by siRNA results in the inhibitory growth of chondrosarcoma cells. (a) Expression of mRNA levels of BMPR2 in HCS-2/8 and SW1353 cells were significantly repressed at 48 h after BMPR2 siRNA transfection. The siBMPR2 transfection efficiency was measured by RT-PCR. (b) The chondrosarcoma cell viabilities decreased sharply when treated with siBMPR2 for 48 h, as assayed by MTS. (c and d) Suppression of BMPR2 significantly decreased capacities of colony formation of HCS-2/8 and SW1353 cells, as analyzed by colony formation assay. (e) Expression of protein levels of BMPR2 in HCS-2/8 and SW1353 cells were suppressed significantly at 48 h after siBMPR2 transfection. Inhibition of BMPR2 resulted in the dephosphorylation of Smad1/5, as assayed by western blot analysis. Data are presented as mean±S.D. (n=3). **P<0.001