Table 2.
Applications of bacterial bioreporters for monitoring organic pollutants in environmental samples.
| Bioreporter
|
Field application
|
||||||
|---|---|---|---|---|---|---|---|
| Host strain | Reporter construct | Analyte | Response time | Detection limit | Matrix | Response time | Reference |
| Burkholderia sartisoli RP007 (pPROBE-phn-luxAB) (Tecon et al., 2010) | phn-luxAB | Naphthalene, phenanthrene | 3 h | 0.17 μM (naphthalene) (Tecon et al., 2010) | Seawater | 3 h | Tecon et al. (2010) |
| E. coli DH5α (pGEc74, pJAMA7) (Sticher et al., 1997) | alkS, PalkB-luxAB | C6-C10 alkane | 15–75 min | 24.5 nM (octane) (Sticher et al., 1997) | Soil (DMSO or water extract) Groundwater Seawater |
1 h 2 h 2 h |
Bundy et al. (2001) Bhattacharyya et al. (2005) Tecon et al. (2010) |
| E. coli DH5α (pHYBP103M3) (Beggah et al., 2008) | hp | 2-hydroxylbiphenyl and biphenyl | 2 h | 0.30 μM (2-hydroxylbiphenyl) (Tecon et al., 2010) | Seawater | 2 h | Tecon et al. (2010) |
| E. coli DH5α (pHYBP109) (Jaspers et al., 2000) | hbpR-PhbpC-luxAB | 2-hydroxylbiphenyl | 2 h | 0.30 μM (2-hydroxylbiphenyl) (Tecon et al., 2010) | Seawater | 2 h | Tecon et al. (2010) |
| E. coli DH5α (pLZCapR) (Shin et al., 2005) | capR-lacZ | Phenolic compounds | 5–7 h | 0.1 μM (phenol) (Shin et al., 2005) | Hospital water Soil (water extract) |
5–7 h 5–7 h |
Shin et al. (2005) Shin et al. (2005) |
| E. coli DH5α (pPROBE-LuxAB-TbuT) (Tecon et al., 2010) | tbuT-luxAB | BTEX | 2 h | 0.24 μM (toluene) (Tecon et al., 2010) | Seawater | 2 h | Tecon et al. (2010) |
| E. coli HMS174 (pOS25) (Selifonova and Eaton, 1996) | ipbR-luxCDABE | Hydrophobic compounds | 100–250 min | Varies among compounds (Selifonova and Eaton, 1996) | Sediment (ethanol extract) Soil (HPCD extract) |
100–250 min 2 h |
Selifonova and Eaton (1996) Diplock et al. (2009) |
| E. coli pGLTUR (Willardson et al., 1998) | xylR-Pu-luc | Toluene | 1 h | 10–20 μM (Willardson et al., 1998) | Soil (ethanol extract) and deep aquifer water Soil (DMSO or water extract) |
1 h 1 h |
Willardson et al. (1998) Bundy et al. (2001) |
| P. fluorescens HK44 (pUTK21) (King et al., 1990) | nahR-PnahG-luxCDABE | Naphthalene | 8–24 min | 12–120 μM (Heitzer et al., 1992) | Soil (DMSO and water extract) Soil Soil (methanol and HPCD extract) |
1 h in situ monitoring 1 h |
Bundy et al. (2001) Ripp et al. (2000) Paton et al. (2009) |
| P. fluorescens OS8 (pDNdmpRlux) (Leedjarv et al., 2006) | dmpR-Po-luxCDABE | Phenolic compounds | 4 h | 0.08 mg/L (phenol), 0.03 mg/L (2-methylphenol) (Leedjarv et al., 2006) | Groundwater and dump leachate | 4 h | Leedjarv et al. (2006) |
| P. putida TVA8 (Applegate et al., 1998) | tod-luxCDABE (Ch) | BTEX | 1–4 h | 0.03–50 mg/L (Applegate et al., 1998) | Soil (HPCD extract) Soil (water extract) Groundwater Wastewater and groundwater |
30 min 2 h 2 h Continuous monitoring |
Diplock et al. (2009) Dawson et al. (2008) Bhattacharyya et al. (2005) Kuncova et al. (2011) |
| Ralstonia eutropha JMP 134-32 (Hay et al., 2000) | tfdRPDII-luxCDABE | 2,4-Dichlorophen-oxyacetic acid | 20– 60 min | 2.0 μM | Soil | 1 h | Toba and Hay (2005) |
Ch: integrated into the chromosome.