Table 4.
Selected environmental applications of eukaryotic bioreporters.
| Bioreporter
|
Field application
|
||||||
|---|---|---|---|---|---|---|---|
| Host strain or cell line | Reporter construct | Analyte | Response time | EC50/Detection limit | Matrix | Response time | Reference |
| S. cerevisiae/AhR (Leskinen et al., 2008) | DRE-luc | AhR ligands | 3.5 h | 5.5 nM DHT/0.5 nM DHT | Sediment | 3.5 h | Leskinen et al. (2008) |
| Rat hepatoma cell line H4IIE DR-CALUX (Murk et al., 1996) | DRE-luc | AhR ligands | 24 h | 0.01 nM TCDD/0.5 fM TCDD | Sediment Soil Indoor dust |
24 h 24 h 24 h |
Dindal et al. (2007), Hilscherova et al. (2010), Houtman et al. (2006), Keiter et al. (2008), Murk et al. (1996), Wolz et al. (2008) Andersson et al. (2009), Sidlova et al. (2009) Suzuki et al. (2010) |
| Human breast carcinoma cell line MDA-kb2 (Wilson et al., 2002) | ARE-luc | Androgenic activity | Overnight | NR/0.1 nM DHT | Water | 24 h | He et al. (2011) |
| S. cerevisiae hERα (Gaido et al., 1997) | ERE-lacZ | Estrogenic activity | 20 h | 3.5 nM DHT/2.19 nM DHT | Wastewater | 20 h | Nakada et al. (2004), Nelson et al. (2007) |
| S. cerevisiae BLYES (Sanseverino et al., 2005) | ERE-lux | Estrogenic activity | 4–6 h | 0.24 nM E2/0.045 nM E2 | Surface water and drinking water | 4–6 h | Bergamasco et al. (2011) |
| S. cerevisiae hERα (Leskinen et al., 2005) | ERE-luc | Estrogenic activity | 2.5 h | 0.5 nM E2/0.03 nM E2 | Wastewater | 2.5 h | Salste et al. (2007) |
| S. cerevisiae hER YES (yeast estrogen screen) (Routledge and Sumpter, 1996) | ERE-lacZ | Estrogenic activity | 72 h | 0.44 nM E2/0.045 nM E2 (Sanseverino et al., 2005) | Wastewater Sediment Surface and ground water Sewage |
72 h 24 h 72–96 h 72 h |
Fernandez et al. (2007), Nelson et al. (2007), Thorpe et al. (2006) Grund et al. (2011) Aneck-Hahn et al. (2008), Beek et al. (2006), Leusch et al. (2010) Leusch et al. (2010) |
| S. cerevisiae hERα (Balsiger et al., 2010) | ERE-lacZ | Estrogenic activity | 2 h | 0.145 nM E2/100–200 pM | Wastewater Sediment |
2 h 2 h |
Balsiger et al. (2010) Jeffries et al. (2011) |
| Human breast carcinoma cell line MCF-7 MELN (Balaguer et al., 1999) | ERE-luc | Estrogenic activity | 16 h | 5 pM E2/1 pM E2 | Sediment Sewage Wastewater Surface and ground water |
16 h 16 h 16 h 16 h |
Louiz et al. (2008) Hernandez-Raquet et al. (2007), Leusch et al. (2010) Cargouet et al. (2004), Dagnino et al. (2010) Cargouet et al. (2007) Cargouet et al. (2004), Leusch et al. (2010) |
| Human breast carcinoma cell line T47D-KBluc (Wilson et al., 2004) | ERE-luc | Estrogenic activity | 24 h | 0.01 nM E2/1 pM E2 | Wastewater Water Sewage |
24 h 24 h 24 h |
Wehmas et al. (2011) He et al. (2011), Leusch et al. (2010) Leusch et al. (2010) |
| S. cerevisiae BMA64/luc (Leskinen et al., 2005) | luc | Toxicity | 0.5 h | NR | Soil | 0.5 h | Lankinen et al. (2011) |
hER: human estrogen receptor; hAR: human androgen receptor; AhR: acyl hydrocarbon receptor; ERE: estrogen response element; ARE: androgen response element; DRE: dioxin response element; E2: 17β-estradiol; T: 17β-testosterone; DHT: dihydrotestosterone; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin; NR: not reported.