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. 2015 Nov 18;6:575. doi: 10.3389/fimmu.2015.00575

Figure 3.

Figure 3

VitD-BMDC have reduced ability to initiate EAE. (A) B10.PL received CFSE-loaded 2 × 106 Tg4.CD45.1 CD4+ T cells 1 day prior to injection of 2 × 104 MBP-loaded Veh-BMDC or VitD-BMDC into the left hindleg. The spleen and right and left popliteal lymph nodes (PLN) were harvested separately and single-cell preparations were made. Plots show CFSE-dilution gated on CD45.1+ T cells from the indicated organs. Results shown are representative of two independent experiments. (B) B10.PLxC57BL/6 mice received 2.5 × 106 Tg4.CD45.1 CD4+ T cells 1 day prior to injection of 2 × 106 MBP-loaded BMDC. Six days later, lymphoid organs were harvested for FACS analysis of total CD4+ and donor CD45.1+ cells. Cell numbers and percentages were compared by a Mann–Whitney U test. (C) 2 × 106 Tg4.CD45.1 T cells were transferred into B10.PLxC57BL/6 mice 1 day prior to injection of 2 × 106 LPS-conditioned, MBP-loaded Veh-BMDC or VitD-BMDC sc in each hindlimb. Pertussis toxin was administered i.p at time of BMDC transfer and 2 days later. Results shown are pooled from three independent experiments.