Figure 5.

VitD-BMDC have a lower proportion of CD11c+ cells. (A) BMDCs were generated in presence of 0, 1, or 10 nM of 1,25(OH)₂D for 9 days. CD11b and CD11c expression was assessed by flow cytometry. (B) Expression of MHC class II, CD40, CD80, and CD86 on Veh-BMDC (black line) or VitD-BMDC (gray shade) gated on CD11c+ cells following overnight maturation without or with LPS. (C) Cytokine production by CD11c+ Veh-BMDC (open bar) and CD11c+ VitD-BMDC (black bar) without or with overnight LPS maturation. (D) 2 × 10⁴ Tg4 CD4+ T cells were cultured with LPS-matured Veh-BMDC (open circle) or VitD-BMDC (black circle) for 72 h and assessed for proliferation and production of IFNγ. Data are from one of four experiments giving consistent results.