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. 2015 Nov 18;6:575. doi: 10.3389/fimmu.2015.00575

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Copyright © 2015 Besusso, Saul, Leech, O’Connor, MacDonald, Anderton and Mellanby.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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VitD-CD11c + BMDC can effectively prime T cells in vivo. B10.PLxC57BL/6 mice received 2 × 10⁶ Tg4.CD45.1 CD4⁺ T cells 1 day prior to s/c injection of 1 × 10⁵ MBP-loaded CD11c+ Veh-BMDC or CD11c+ VitD-BMDC into the hindlimb. Six days later, spleen and draining lymph nodes were harvested for FACS analysis of total CD4⁺ and donor CD45.1⁺ cells. The total numbers of cells, number of donor Tg4 cells, percentage of donor Tg4 cells of total CD4+ cells, percentage of Ki67+ of donor Tg4 cells, and percentage of GM-CSF+ donor Tg4 are shown in spleen and DLN. Data are from one of two experiments giving consistent results.