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. Author manuscript; available in PMC: 2016 Apr 15.
Published in final edited form as: Nature. 2015 Oct 15;526(7573):385ā€“390. doi: 10.1038/nature15700

Figure 4. MCM ablation does not affect other male-specific behaviours.

Figure 4

Efficiency of intact and MCM-ablated males [inIs179(ida-1prom::gfp);him-8(e1489) or otIs356(rab-3prom::rfp)him-5(e1490)], and wild-type [him-5(e1490)] and pdf-1(tm1996) mutant males in male-specific behaviours.

a, Response to wild-type or daf-22 (m130) hermaphrodite-conditioned media and purified ascaroside pheromones (80nM Ascr#3, 800nM Ascr#2). Graphs represent Tukey box plots of logarithmic transformations of the data; n= number of independent events (i.e. entry in scoring region); T-test with Bonferroni correction was used for statistical analysis. ***P<0.001; *P<0.05; n.s.= no statistically significant difference (pā‰„0.05).

b, Summary diagram of the data plotted in a. Statistical significance compared to control buffer is indicated by +++, P<0.001; +, P<0.05; āˆ’ = no statistically significant difference. n.d., not determined.

c and d, Execution of mating sub-steps (c) and exploration in search of mates (d). +++ indicates performance level of intact, control males.