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DI fusion protein sequence showing the N-terminal hexa-histidine tag (italics), the BirA recognition site for potential biotinylation (boxed) and the restriction protease FXa cleavage site (underlined). The cleavage site is indicated with an arrow and the first amino acid of DI is bolded. The fusion protein is 12.9 kDa while native DI which contains 64 amino acids is 7.2 kDa
